Why did the original “one-gene, one-enzyme” hypothesis have to be modified?
A) Some RNAs have catalytic activity.
B) Not all proteins are coded by genes.
C) Some enzymes are made up of more than one polypeptide.
D) All genes code for multiple enzymes.
E) Not all enzymes are coded for by genes.
C) Some enzymes are made up of more than one polypeptide.
Which component is required for the initiation of transcription?
A) The start codon AUG
B) Promoter DNA sequence
C) Ribonucleoside triphosphates
D) Terminator DNA sequence
E) DNA polymerase
B) Promoter DNA sequence
Put the following four steps of eukaryotic gene expression in order, from beginning to end.
(1) Pre-mRNA is processed to make mRNA.
(2) Ribosomes translate the mRNA sequence to make proteins. (3) mRNA is transported to the cytoplasm.
(4) DNA is used as a template to make pre-mRNA.
A) 1; 4; 3; 2
B) 4; 3; 1; 2
C) 4; 1; 3; 2
D) 4; 1; 2; 3
E) 1; 2; 4; 3
C) 4; 1; 3; 2
RNA polymerases differ from DNA polymerases in that
A) RNA polymerases do not require a template.
B) RNA polymerases use deoxyribonucleoside triphosphates as substrates.
C) DNA polymerases use ribonucleoside triphosphates as substrates.
D) RNA polymerases are less effective at proofreading than DNA polymerases.
E) only DNA polymerases are processive.
D) RNA polymerases are less effective at proofreading than DNA polymerases.
Which statement about pre-mRNA splicing is false?
A) It removes introns.
B) It is performed by small nuclear ribonucleoprotein particles (snRNPs).
C) It is common in prokaryotes.
D) It is directed by consensus sequences.
E) It shortens the RNA molecule.
C) It is common in prokaryotes.
Which component of transcribed RNA in eukaryotes is present in the primary transcript but is removed before translation occurs?
A) Intron
B) 5 cap
C) Ribosome binding site
D) Poly A tail
E) Exon
A) Intron
At the initiation complex of translation in eukaryotes,
A) the complex of mRNA and the large ribosomal subunit are formed.
B) the anticodon of tRNA charged with methionine binds to mRNA associated with
the small ribosomal subunit.
C) the large ribosomal subunit binds to mRNA, causing the release of the small
subunit.
D) the large ribosomal subunit binds to the complex before the methionine-charged
tRNA.
E) the poly A tail of mRNA is directly involved
B) the anticodon of tRNA charged with methionine binds to mRNA associated with
Which component is not involved in the elongation step of translation?
A) Peptidyl transferase
B) Charged tRNA
C) Ribosomes
D) The amino acid site
E) RNA polymerase
E) RNA polymerase
Termination of translation requires
A) a termination signal, RNA polymerase, and a release factor.
B) a release factor, initiator tRNA, and ribosomes.
C) initiation factors, the small subunit of the ribosome, and mRNA.
D) elongation factors and charged tRNAs.
E) a stop codon positioned at the A site of the ribosome and a release factor.
E) a stop codon positioned at the A site of the ribosome and a release factor.
The enzyme that catalyzes the synthesis of RNA is
A) peptidyl transferase.
B) DNA polymerase.
C) tRNA synthase.
D) ribosomal RNA.
E) RNA polymerase.
E) RNA polymerase.
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