Hans Coaching Christian Gram 1883, a Danish Physician, while attempting to differentiate bacteria from human tissue by different staining methods. He noted differences in stain retention by bacteria due to the complex differences in the component and structure of their cell wall. Gram’s Stain, widely used method of staining bacteria as an aid to their identification.
In Gram’s method, bacteria are first stained with gentian violet (a dye consisting of a methyl derivative of paraprofessional) and then treated with Gram’s solution, consisting of 1 part iodine, 2 parts potassium iodide, and 300 parts water. After being washed with ethyl alcohol, the bacteria will either retain the strong blue color of gentian violet or be completely decolonize. Sometimes a counteraction such as fuchsias or eosin is applied to give the decolonize bacteria a reddish color to make them more visible.
Bacteria that retain the blue stain are known as gram- positive; those that do not are known as gram-negative. Organisms that sometimes retain the blue color and sometimes do not are known as gram-variable. Typical gram-positive bacteria are those staphylococci that produce boils; typical gram- active bacteria are the bacilli that cause whooping cough; typical gram-variable bacteria are the bacilli that cause tuberculosis. The littérateurs and chemical composition of the cell walls of Gram-positive and Gram negative bacteria are quite different.
Nonprofit,the cell wall of Gram- positive bacteria reveals a single thick and more or less homogeneous layer, whereas Gram-negative bacteria have a thinner,distinctly layered cell wall with an outer membrane resembling the typical terminal cytoplasm membrane. The polymers found in the cell walls of these two groups of bacteria are chemically quite different. The walls of Gram-negative cells are mainly composed of alphanumerically, phosphoric ,protein, lepidopterist, and relatively little pedagogical(salesmanship’s% of the total cell wall).
The Gram- positive cells contain pedagogical(salutatorian’s%photochemical wholeheartedness’s or technocratic(robots),or technocracies, as major components. Thus, in contrast to the Gram-negative bacteria, the Gram-positive bacteria contain hardly any lipids in their cell walls. There is, however, one exception: acid-fast bacteria. They are resistant to decentralization with acidic ethanol after tanning with fuchsia (Zilch-Nielsen staining).
These acid-abstracter (Mycobacterium, Anaconda, and Crematoriums constricted) are Gram-positive bacteria which contain large amounts of lipids in their cell walls; nonpolitical, monosyllabic(high-molecular- weight,3-hydroxides with a long alkyl branch Gram staining classification of bacteria The Gram staining technique attempts to classify bacteria into two wide classes based on their reaction to the stain when viewed under a bright field microscope, it is important to note that this system of classification is based on bacterial cell wall intent ,natural classification scheme that reflects major differences in cell wall structure and to some extent the mechanisms involved in disease. Gram positive: The Gram positive bacteria appear purple in color under the bright field microscope when viewed with appropriate magnification. They have a single membrane consisting of a thick pedagogical layer – no alphanumerically.
Liposuction acids and wall tectonic acids make the surface of Gram-positive bacteria negatively charged Gram negative: The Gram negative bacteria appear purple in color under the bright field microscope when viewed with appropriate magnification. Hey posses inner and outer membranes, with outer membrane having alphanumerically molecules . They are characterized by Relatively thin pedagogical layer Outside of the pedagogical layer is an outer membrane characterized by unique polymers – Alphanumerically (LAPS) extending from the outer surface – Lepidopterist anchoring the outer membrane to the pedagogical layer – Poring that facilitate transport of large molecules across the outer membrane.