How effective is Also in the reduction of bacterial growth compared to Penises in reduction of E. Coli growth in agar at room temperature? Background Information: Penises and Also are both common household disinfectants that make very big commercial claims; both claim to kill 99. 9 percent of bacteria. Also contains Phenol which is a highly volatile organic compound. Phenol is a carbolic acid found in some household cleaning supplies. Phenol is very toxic and because of this it is designed to kill bacteria.
The phenol within the Also causes the environment where he bacteria thrive to be uninhabitable. Penises has several other organic compounds in it as well but none as volatile as Phenol, yet some consumers say that Penises is Just as useful as Also. Penises contains alkyl and sheepishly school which are both do not disrupt the environment of the bacteria as effectively. Variables Measurement range Independent Also and penises Concentration 2-3 millimeters dependent Bacterial growth colonies 5-7 colonies Controls Uses how Agar plate The same agar allows this specific type of bacteria to grow; and adds consistency to environment.
Use same type of agar plates. Moisture Allows researcher to know how bacteria reacts with condensations Use same incubator tort storage temperature Adds consistency to environment and gives bacteria optimum environment for growth. Use same incubator for storage Type of bacteria Adds consistency for experiment and is a bacterium Use same type of bacteria (E. Coli) Hypothesis: I think the Phenol in the Also will cause the most significant reduction in bacterial growth, because the Phenol is a volatile organic compound capable of bacterial reduction in extensive amounts.
Materials: 1 bottle of Also 1 bottle of Penises 2 graduated cylinders agar plates sterilized cottonwoods incubator rods 2 Petri dishes Procedure: Wash your hands 2) Label the top of the container with your initials and name of household cleaner that is going to be used on the Streptococcus Bacillus. 3) Begin by sterilizing the Petri dishes. Accomplish by placing them in a bath of boiling water and allowing to dry upside down on a sanitized drying rack or lab work bench. If the Petri dishes are still enclosed in the original packaging, leave them until ready to use.
For 500 millimeters (ml) of agar mixture, you can fill 25 average-sized Petri dishes. 4) Prepare agar powder by placing it in the microwave with the appropriate amount of tater. The label should provide specific directions although 6. 9 grams to 500 ml of water (or Just a little more than two cups) is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard. 5) Treat bottled agar formula carefully by loosening the bottle cap (but not removing) before placing in the microwave to warm and soften into a liquid state.
Sterilize the bottle after microwaving by placing the neck of the bottle over an open flame a few times to ensure any airborne germs are eradicated before proceeding to plate making. 6) Place the Petri dishes the right way up and crack but do not remove the lids. This is to prepare them for individual handling when the other hand is holding the agar mixture in a suitable pouring vessel such as a glass pitcher. 7) Gently lift the lid of the Petri dish with one hand and hover the lid immediately over the bottom dish to remove any chance of airborne germs entering. ) Pour an amount of agar liquid into each dish as you hover the lid as mentioned in the previous step. About 1/8 inch is a standard thickness for the liquid measure. 9) Allow the agar to set into the dishes by leaving them on a TLA, clean surface to dry in the natural room temperature. Secure lids once set and store upside down. After agar plates are prepared and ready to use . Take the sterile cottons out of the wrapping. Place sterile cottons in the Streptococcus bacterial culture, and rotate the cottons in a clockwise motion for 25 seconds .
Extract the cottons carefully from the bacterial culture slowly and carefully Wipe the extracted cottons making sure you do not press to hard as to make on impression in the agar. Wipe the cottons from one end of the plate to the other making a zigzag all the way down the Plate. Throw away the cottons in hazardous waste bin Seal the plate and place the place agar plate having the agar facing downward in incubator at 98*F . Wait 24 hours 17) After 24 hours, get a metal rod with a loop at the end and turn on the Bunsen burner . Put the end of the metal rod with the loop on the Bunsen burner and keep it there for 10 seconds.