The universe population is increasing twenty-four hours by twenty-four hours and is expected to make 8.5 billion by 2025. At the other terminal environmental emphasis ( biotic and abiotic emphasis ) destroys the overall output of the agricultural harvests. Plants are subjected to assortment of abiotic emphasiss like high temperature, cold, drouth, salt, UV. However, amongst these emphasiss, salt is considered the most confining factor for productiveness of agricultural harvests. Salinity is a worldwide job that limits distribution and production of major harvests. Salt emphasis in dirt or H2O is one of the major emphasiss particularly in waterless and semi-arid parts ( Allakhverdier et al. 2000 ; Koca et Al. 2007 ) . One-fifth of irrigated agribusiness is adversely affected by dirt salt ( Flowers and Yeo, 1995 ) . Salinity is responsible for the initiation of primary effects like ionic emphasis osmotic emphasis which in bend induces secondary emphasiss such as oxidative emphasis. Oxidative emphasis leads to the accretion of reactive O species ( ROS ) which are really unsafe to workss and in higher concentrations leads to decease of the works cells ( Ahmad and Sharma, 2008 ; Ahmad et al. , 2008 ; 2010a, B, degree Celsius ) . It causes oxidative harm to biomolecules like membrane lipoids, proteins, and nucleic acids ( Hernandez et al. 2001 ) .
Plants have the mechanisms to antagonize the hurtful effects of primary and secondary emphasiss ( Devi and Prasad, 1998 ) through the initiation of osmolytes and antioxidants ( Ashraf and Foolad, 2007 ; Foyer et al. , 1997 ; Ahmad et al. , 2008 ; 2010a, B, degree Celsius ) . The antioxidant enzymes include superoxide dismutases, peroxidases, catalases and glutathione reductases. The superoxide anion, which is most unsafe reactive O species, is scavenged in workss by superoxide dismutase, which converts superoxide anion to hydrogen peroxide ( Alscher et al. , 2003 ) . Hydrogen peroxide ( H2O2 ) is scavenged straight by catalase, which converts it to H2O and molecular O. Measurement of activities of antioxidant enzymes can therefore be used to bespeak oxidative emphasis in workss ( Geebelen et al. , 2002 ) .
Brassica juncea ( mustard ) , a works within the Brassicaceae household, is an of import oil seed harvest grown extensively in waterless and semi-arid parts ( Singh et al. 2001 ) . India is 2nd largest manufacturer of rapeseed mustard ( Afroz et al. , 2005 ) . However, Indian mustard production still remains deficient to run into even the day-to-day demand of its people ( Khan et al. , 2002 ) . So it becomes necessary to works life scientists to increase the production of mustard within the given fortunes. The present survey was undertaken to look into the consequence of NaCl on Biomass, biochemical properties, lipid peroxidation and the activity of some cardinal antioxidant enzymes in mustard cultivars and take us to bring forth the salt tolerant cultivars to reclamate the salt affected land.
MATERIALS AND METHODS
Seeds of Brassica juncea were sown in earthen pots incorporating 5 kilogram of peat, perlite, and sand ( 1:1:1, v/v/v ) . After sprouting they were transferred to one works per pot and grown for farther three hebdomads under natural photoperiod of 12-13 H and temperature of 28A±40C. The workss were treated with different concentrations of NaCl: 100 and 200 millimeter. The experiments was laid out in a wholly randomized design with five replicates. The samples were harvested at 45 yearss after intervention.
Growth, gas exchange, Chlorophyll fluorescence and pigment concentration
For dry weight ( DW ) finding, foliages and roots were separated and were dried at 700C for 48 H and weighed.
Measurements of net CO2 assimilation rate ( A ) , stomatous conductance ( gs ) and transpiration ( E ) rate were made on a to the full expanded youngest foliage of each works utilizing an unfastened system LCA-4 ADC portable infrared gas analyser ( Analytical Development Company, Hoddesdon, England ) . These measurings were made from 10:30 to 12:00 hours.
Chl fluorescence measurings were made in affiliated foliages in the growing chamber with a PAM 2000 setup ( H. Walz, Effeltrich, Germany ) . The maximal efficiency of PSII photochemistry under dark-adapted ( Fv/Fm ) , quantum output of PSII ( I¦psII ) , nonphotochemical extinction ( NPQ ) and photochemical extinction coefficient ( qP ) were calculated harmonizing to the method of Li et Al. ( 2007 ) .
Chlorophyll content was determined by the method of Hiscox and Israelstam ( 1979 ) . Fresh stuff ( 100 milligram ) was kept in an extraction reagent dimethyl sulfoxide ( DMSO ) . Tubes were kept in oven at 650C for 40 min. 1 milliliter aliquot was assorted with 2ml DMSO and so vortexed. Absorbance was determined spectrophotometerically at 480, 510, 645, 663 nanometer ( Beckman 640 D, USA ) utilizing DMSO for a space.
RWC, electrolyte escape % and Proline content
Leaf comparative H2O content ( RWC ) was measured in to the full expanded foliages of four workss per replicate. Five leaf phonograph record of 10 millimeters diameter were excised from the interveinal countries of each Leaf. For each replicate, 20 phonograph record were pooled and their FW determined. The foliage phonograph record were floated on deionised H2O for 7 Hs under low irradiance and so the bombastic weight ( TW ) recorded. Then the samples were dried at 80 oC for 24 H to find the ( DW ) . The trials showed that complete hydration of the foliage phonograph record occurred within 4 h. The comparative H2O content ( RWC ) was calculated utilizing the undermentioned expression ( Smart and Bingham, 1974 ) :
RWC ( % ) = ( FW-DW/TW-DW ) X 100
The entire inorganic ions leaked out in the foliages under salt emphasis were measured as described by Dionisio-Sese and Tobita ( 1998 ) . Twenty leaf phonograph record were taken in a boiling tubing incorporating 10A milliliter of deionized H2O and electrical conduction ( EC ) was measured ( ECa ) . The contents were heated at 50 and 60A A°C for 25A min each in a H2O bath and EC was measured ( ECb ) . Subsequently, the contents were boiled at 100A A°C for 10A min and the EC once more recorded ( ECc ) . The electrolytic escape was calculated utilizing the expression:
Proline concentration in the foliages was detemined spectrophotometerically following Bates et Al. ( 1973 ) . Fresh stuff ( 300 mg each sample ) was homogenized in 10 milliliter of 3 % aqueous sulfosalicylic acid. The homogenate was centrifuged at 12000 revolutions per minute for 15 min. 2 ml aliquot of the supernatant was assorted with an equal volume of acetic acid and acid ninhydrin and incubated for 1 H at 100 0C. The reaction was terminated in an ice bath and extracted with 4 milliliters of methylbenzene. The infusion was vortexed for 20 s. The chromatophore-containing methylbenzene was so aspirated from the aqueous stage, and its optical density determined spectrophotometerically at 520 nanometer ( Beckman 640 D, USA ) utilizing methylbenzene for a space.
Determination of H2O2 content and lipid peroxidation ( MDA )
The H peroxide content was determined harmonizing to Velikova et Al. ( 2000 ) . Fresh works stuff ( 500 milligram ) was homogenized with 5 milliliters of 0.1 % ( w/v ) trichloroacetic acid ( TCA ) . The homogenate was centrifuged at 12,000 revolutions per minute for 15 min, and 0.5 milliliter of the supernatant was added to 0.5 milliliter of 10 millimeters potassium phosphate buffer ( pH 7.0 ) and 1 milliliter of 1 M K iodide ( KI ) . The optical density of the supernatant was measured at 390 nanometer. The content of H2O2 was calculated by comparing with a standard standardization curve, antecedently plotted by utilizing different concentrations of H2O2.
Lipid peroxidation was determined by mensurating malondialdehyde ( MDA ) formation harmonizing to Madhava Rao and Sresty ( 2000 ) . Fresh foliages ( 500 milligram ) were homogenized with 2.5 milliliters of 0.1 % trichloroacetic acid ( TCA ) . The homogenate was centrifuged for 10 min at 10,000 revolutions per minute. For every 1mL of the aliquot, 4 milliliter of 20 % TCA incorporating 0.5 % thio barbituric acid ( TBA ) were added. The mixture was heated at 95 oC for 30 min and so cooled quickly on an ice bath. Afterwards, the mixture was centrifuged for 15 min at 10,000 revolutions per minute and the optical density of the supernatant was measured at 532 nanometer. Measurements were corrected for broad turbidness by substracting the optical density at 600 nanometer. The concentration of MDA was calculated utilizing an extinction coefficient of 155 mMa?’1 cma?’1.
Extraction of the enzymes
All experiments were performed at 4A A°C. The 10A g foliage were homogenized with 50 volumes of 100A mM Tris-HCl ( pH 7.5 ) incorporating 5A mM DTT, 10A millimeter MgCl2, 1A millimeter EDTA, 5A millimeter Mg ethanoate and 1.5 % PVP-40. After filtration in cheesecloth the homogenate was centrifuged at 10,000 revolutions per minute for 15A min. The supernatant collected was used as a beginning of enzyme. Serine and cysteine protease inhibitors ( 1 millimeter PMSF + 1 I?g/ml aproptinin ) was besides added in the extraction buffer. For mensurating APX activity the tissue was individually ground in homogenising medium incorporating 2.0 mM ascorbate in add-on to other ingredients. The soluble protein content was determined by Bradford ( 1976 ) with standard curves prepared utilizing bovine serum albumen.
Superoxide dismutase ( SOD, EC 188.8.131.52 ) activity was determined by the method of Van Rossun et Al. ( 1997 ) by following the photoreduction of nitroblue tetrazolium. The reaction mixture contained: 50A millimeter phosphate buffer ( pH 7.8 ) , 0.1A millimeter EDTA, 13A millimeter methionine, 75A I?M nitroblue tetrazolium ( NTB ) , 2A I?M vitamin B2 and 100A I?l of the supernatant. The reaction was initiated by puting the tubings under two 15A W fluorescent lamps. The reaction was terminated after 10A min by taking the reaction tubings from the light beginning. Non-illuminated reactions mixture served as a space. The optical density of the reaction merchandises were measured at 560A nanometer. SOD activity was expressed as Unit mg-1 protein. One unit of SOD was defined as the sum of protein doing a 50 % lessening of the SOD-inhibitable NBT decrease.
A method of Luck ( 1974 ) was employed for the check of catalase ( CAT, EC 184.108.40.206 ) . The enzyme infusion ( 50A I?l ) was added to 3A milliliter of 20 millimeters hydrogen peroxide and 50 millimeter phosphate buffer ( pH 7.0 ) solution. The lessening in optical density was measured at 240 nanometer. The enzyme activity was calculated utilizing the extinction coefficient of 36×103 mM-1 cm-1 and expressed as unit mg-1 protein.
Ascorbate peroxidase ( APX, EC220.127.116.11 ) was spectrophotometrically assayed following a lessening in the optical density at 265A nanometer ( Nakano and Asada, 1981 ) . The 3 milliliters assay mixture contained: 0.1 millimeter EDTA, 0.5A millimeter ascorbate and 0.1A millimeter H2O2 in 50A mM K phosphate buffer ( pH 7.0 ) with 0.1 milliliter of the enzyme infusion. The H2O2 dependent oxidization of ascorbate was followed by a lessening in the optical density in 290 nanometer. APX activity was expressed as unit mg-1 protein.
Glutathione reductase ( GR, EC 18.104.22.168 ) activity was determined by following the rate of NADPH oxidization as measured by the lessening in the optical density at 340A nanometer ( Carlberg and Mannervik, 1985 ) . The assay mixture ( 1A milliliter ) contained: 0.75 I?l K phosphate buffer ( pH 7 ) with 2A millimeters EDTA, 75A I?M NADPH ( 2 millimeter ) , 75A I?l GSSG ( 20 millimeter ) . Chemical reaction was initiated by adding 0.1 ml enzyme infusion to the mixture and the lessening in optical density was measured at 340 nanometer for 2 min. GR activity was calculated with the extinction coefficient of NADPH of 6.2 mM-1 cm-1 and expressed as I?molNADPH oxidized min-1 ( Units mg-1 protein ) .
Datas for each variable were subjected to one manner analysis of discrepancy ( ANOVA ) . Duncan ‘s Multiple Range Test ( DMRT ) at 5 % chance was employed for measuring the important differences among the average values of different properties. The values are agencies of five reproductions.
Salt emphasis decreases the biomass of the mustard cultivars and the consequences are depicted in fig.1 ( A-D ) . Shoot and root length was observed to diminish but the lessening was more marked in Rohini as compared to RH-30 and Varuna. A important lessening in shoot dw of 31.4 % , 37.3 % and 45.7 % was observed in Varuna, RH-30 and Rohini severally at 200 millimeter of NaCl emphasis as compared to command. Root dw besides showed the same diminishing tendency ( fi. 1, C & A ; D ) .
CO2 assimilation rate ( A ) decreases from 14.5 % to 21.8 % at 100 and 200 millimeter NaCl emphasis in Varuna severally. Same diminishing tendency was observed in RH-30 ( 26.8 % ) and Rohini ( 42.2 % ) at 200 millimeter NaCl emphasis ( fig.2, A ) .
Stomatal conductance ( gs ) and transpiration rate ( E ) besides decreases in all the cultivars at all emphasis governments but more lessening was observed in Rohini and RH-30 as compared to Varuna ( fig. 2, B & A ; C ) .
A non-significant lessening was observed in Fv/Fm in all the cultivars of mustard at 100 millimeter NaCl emphasis. But at higher concentration of salt ( 200 mM NaCl ) a lessening in Fv/Fm, I¦psII and qP accompanied with addition in NPQ was observed ( table 1 ) .
Salt emphasis decreases chlorophyll content ‘a ‘ , ‘b ‘ and ‘a/b ‘ ratio in all the cultivars of mustard. A important ( pa‰¤ 0.5 ) lessening in chlorophyll ‘a ‘ was noticed in all the cultivars and more marked lessening ( 28.9 % ) was shown by Rohini at 200 millimeter NaCl emphasis ( fig. 3, A ) .
A important lessening in chlorophyll ‘b ‘ was observed between Rohini and Varuna but non important lessening was observed between RH-30 and Rohini ( fig. 3, B ) . same diminishing tendency was besides observed in chl. a/b ratio ( fig. 3, C ) .
The consequences related to the consequence of NaCl on RWC is presented in fig. 3, D. As the concentration of NaCl increase the RWC lessenings and the lessening was more marked in Rohini ( 46.8 % ) than RH-30 ( 40 % ) and Varuna ( 26.3 % ) at 200 millimeter NaCl emphasis.
A really high electrolyte escape was observed in all the three cultivars of mustard particularly at 200 millimeter NaCl emphasis. A lessening of 62.9 % , 67 % and 67.5 % was observed in Varuna, RH-30 and Rohini severally at 200 millimeter NaCl emphasis ( fig. 3, E ) .
Proline was observed to increase at all emphasis degrees in all the cultivars of mustard. The order of addition in proline was 59.1 % , 53.8 % and 46.8 % by Varuna, RH-30 and Rohoni severally at 200 millimeter NaCl emphasis ( fig. 3, F ) .
Varuna showed addition of H2O2 from 41.1 % to 51.2 % , RH-30 55.4 % and 64.9 % and Rohini showed 55.3 % to 64.4 % during 100 and 200 millimeter NaCl emphasis severally ( fig. 4, A ) .
The MDA concentration was observed more in Rohini ( 45.9 % ) and RH-30 ( 45.7 % ) than Varuna ( 34.6 % ) ( fig. 4, B ) .
SOD activity in the shoots of all three cultivars increased due to salt emphasis and the cultivars differed significantly for this property. Cultivar Varuna had the highest Turf activity followed by curriculum vitae. RH-30, but the lowest Turf activity was recorded in curriculum vitae. Rohini ( fig. 4, C ) .
A important addition in shoot catalase activity was observed in all three cultivars under salt emphasis. However, curriculum vitae. Rohini had the lowest shoot catalase activity under saline conditions. In contrast, RH-30 and Varuna were the highest in catalase activities under salt emphasis ( fig. 4, D ) .
A important addition in shoot APX activity was observed in all three cultivars under salt emphasis. Cultivars differed significantly in peroxidase activity under both control and saline conditions. Highest activity of the enzyme was recorded in curriculum vitae. Varuna and RH-30 under both saline and control conditions, but this addition was non marked in curriculum vitae. Rohini under saline conditions ( fig. 4, E ) .
All the three cultivars of mustard showed increased activity of GR under saline conditions. Highest GR activity was observed in Varuna and RH-30 as compared to Rohini under saline conditions ( fig. 4, F ) .
Our consequences of diminishing biomass corroborates with the findings of Zribi et Al. ( 2009 ) who showed that addition in salt concentration decreases the biomass of tomato workss. Similar consequences have been reported by Akram et Al. ( 2009 ) in helianthus, Mohammad et Al. ( 1998 ) in tomato, Chartzoulakis and Klapaki ( 2000 ) in Piper nigrum, Imada and Tamai ( 2009 ) in thickly settled alba, Keutgen and Pawelzik ( 2009 ) in strawberry and Ahmad and Sharma ( 2010 ) in mulberry. The reduced biomass in the high-salt intervention resulted in the smaller accretion of foods. Increased salt degrees under natural conditions may therefore take to terrible growing decreases and deter constitution of the species. Cell division and cell elongation is reduced due to NaCl emphasis which in bend inhibits growing ( Yasseen et al. , 1987 ) .
Salt emphasis decreases all gas exchange parametric quantities such as CO2 assimilation rate ( A ) , transpiration rate ( E ) , stomatous conductance ( gs ) significantly. Similar studies have been reported by Hameed and Ashraf ( 2008 ) in Cynodon dactylon, Akram et Al. ( 2008-09 ) in helianthus, Zheng et Al. ( 2009 ) in wheat and Ahmad and Sharma ( 2010 ) in mulberry. A similar study is besides observed by Yang et al. , ( 2009 ) in Populus cathayana and Zribi et Al. ( 2009 ) in tomato. Leaf stomatous conductance decreased significantly with increasing NaCl concentration ( Zribi et al. 2009 ; Ahmad and Sharma, 2010 ) . A, gs and Fv/Fm decreased in analogue during salt emphasis. Stomatal closing might be one of the factors, which are responsible for the decrease in A. The salt induces ABA accretion and causes stomatous closing ( Aldesuguy and Ibrahim, 2001 ) .
No important impact on Fv/Fm was observed in the mild-stressed works, but a important lessening was found under terrible emphasis and the consequences are in conformity with the findings of Zribi et Al. ( 2009 ) in tomato. Similar consequences have been reported by Everard et Al. ( 1994 ) ; Lu et Al. ( 2003 ) Cha-um and Kirdmanee ( 2009 ) and Yang et al. , ( 2009 ) . Akram et Al. ( 2009 ) besides showed that salt emphasis reduced the quantum output ( Fv/Fm ) of photosystem-II ( PSII ) . During terrible salt intervention a lessening in photochemical procedures along with, a important addition in non-photochemical extinction ( NPQ ) was observed in tomato ( Zribi et al. 2008-2009 ) and wheat ( Zheng et al. 2009 ) . A sustained lessening of the Fv/Fm may bespeak the happening of photoinhibitory harm ( Maxwell and Johnson, 2000 ; Colom and Vazzana, 2003 ) . A correlativity of Na+ accretion in foliages and qp, NPQ, I¦PSII have been found by many workers and these parametric quantities could be considered as an index of photosynthetic perturbation in workss under salt emphasis ( Zribi et al. ( 2008-2009 ) .
In the present survey salt stress decreases the RWC in all cultivars of mustard. Yang et al. , ( 2009 ) have observed the same lessening in Populus cathayana during salt emphasis. Decrease in RWC under salt emphasis was besides detected in Populus ( Eitel et al. 2006 ) , in olive tree ( Boussadia et al. 2008 ) in pea ( Ahmad and Jhon, 2005 ) and in mulberry ( Ahmad and Sharma, 2010 ) . The lessening in RWC suggests that salt caused H2O shortage in workss. The negative consequence on works H2O dealingss was induced by an addition in soluble salts, which slowed down the consumption of H2O and foods, doing osmotic effects and toxicity. Duan et Al. ( 2005 ) have reported that reduced foliage RWC is positively correlated with decreased photosynthetic rates.
Salt emphasis decreases the chlorolophyll content have been reported by many workers ( Zheng et al. , 2009 ; Hameed and Ashraf 2008 ; EfeoAYlu et al. , 2009 ; Cha-um and Kirdmanee 2009 ; Yang et al. , 2009 ; Ahmad, 2010 ; Ahmad and Jhon, 2005 ) . The lessening in Chla and Chlb, might be due to either slow synthesis or fast dislocation of chlorophyll pigments under salt emphasis ( Ashraf, 2003 ) . Salt hampers the consumption of foods from the dirt that might diminish the pigment concentration.
Leaf proline content increased significantly in Cynodon dactylon ( Hameed and Ashraf 2008 ) during salt emphasis. Cha-um and Kirdmanee ( 2009 ) besides showed that increasing concentration of salt increases the proline content in sugar cane. Proline content have been reported to increase during other emphasiss ( EfeoAYlu et al. , 2009 ; Ahmad et al. , 2006 ; Ahmad, 2010 ; Ahmad et al. , 2010a, B ; Ahmad and Sharma, 2010 ) . The synthesis of proline is widely used by workss to stabilise membranes and keep the conformation of proteins at low leaf H2O potencies. Proline is besides known to be involved in cut downing the exposure harm in the thylakoid membranes by scavenging and/or cut downing the production of 1O2 ( Reddy et al. , 2004 ) .
H2O2 content were much higher under salt emphasis than the control in wheat ( Zheng et al. ( 2009 ) , Yang et al. , ( 2009 ) in Populus cathayana. Salt tolerant cultivars of mulberry accumulates less H2O2 as compared to salt sensitive cultivar ( Ahmad et al. , 2010a ) . Jaleel et Al. ( 2007 ) besides demonstrated that NaCl increased H2O2 in all parts of Catharanthus roseus workss. The addition in H2O2 in bend increases the lipid peroxidation that leads to the escape of the membranes.
MDA content significantly increased under the salt intervention and as compared with the control in wheat ( Zheng et al. ( 2009 ) . Yang et al. , ( 2009 ) besides observed addition in MDA content in Populus cathayana. Salt sensitive cultivars shows more lipid peroxidation as compared to tolerant cultivars of mulberry ( Ahmad et al. , 2010a ) . The additions in electrolyte escape, MDA and H2O2 content suggested that salt-stressed workss encountered cellular harm and lipid peroxidation. Similar consequences have been reported by Lei et Al. ( 2007 ) . our earlier work on pea besides confirms the same consequences ( Ahmad et al. , 2008 ) . Membrane lipid peroxidation, has frequently been used as a tool to measure the grade of works sensitiveness to emphasize.
The consequences related to the alteration in antioxidants ( SOD, CAT, APX and GR ) in the present survey corroborates with the findings of Ashraf and Ali, 2008 in canola ( Brassica napus ) under saline conditions. Our increased antioxidant activity besides corroborates with the findings of Yang et al. , ( 2009 ) who besides recorded the increased CAT, APX, GR in Populus cathayana during salt emphasis. Experimental consequences showed addition in antioxidant activity under salt emphasis, in pea, maize, tea, mustard and mulberry ( Ahmad et al. 2008 ; Tuna et Al. 2008 ; Upadhyaya et Al. 2008 ; Ahmad, 2010 ; Ahmad et al. , 2010a, B ) . Antioxidant enzymes are known to cut down the degrees of superoxide and H peroxide in workss ( Ali and Alqurainy, 2006 ) . The first line of defense mechanism in oxidative emphasis is superoxide dismutase that converts O2a?’aˆ? to H2O2. Catalases are the chief scavenging enzymes which can straight dismutate H2O2 and is indispensable for ROS detoxification during emphasis ( Van Breusegem et Al. 2001 ) . This is besides due to the fact that there is proliferation of peroxisomes during emphasis, which might assist in scavenging of H2O2 that diffuse from the cytosol ( Lopez-Huertas et Al. 2000 ) . Increase in catalase activity is supposed to be an adaptative trait perchance assisting to get the better of the harm to the tissue metamorphosis by cut downing toxic degrees of H2O2 ( Sekmen et al. 2007 ; Vital et Al. 2008 ) . Ascorbate peroxidase is one of the most of import antioxidant enzymes of workss involved in scavenging of H2O2 in water-water and ascorbate-glutathione rhythms and utilizes AsA as the negatron giver. APXs cut down H2O2 to H2O and play an of import function in the antioxidant system of workss ( Kangasjarvi et al. 2008 ) . GR catalyses the NADPH-dependent decrease of oxidised glutathione ( GSSG ) to its reduced signifier ( GSH ) . GR activity is thought to increase the ratio of NADP+/NADPH, and the NADP+ accepts negatrons from the photosynthetic negatron conveyance concatenation. Thus the flow of negatrons to O2 and hence, the formation of O2a?’ can be minimized.
In decision, differences in biomass output, leaf H2O position, photosynthetic public presentation, pigment content, proline accretion, H2O2 content, lipid peroxidation and antioxidative enzyme activity between Brassica juncea cultivars were observed in the present survey. Of all cultivars, Varuna were comparatively higher in shoot fresh and dry weight, proline, SOD, APX, CAT and GR activities than the other cultivars of mustard, while RH-30 and Rohini were higher in electrolyte escape, H2O2 and MDA degrees. The differential salt tolerance of the mustard cultivars was found to be associated with higher antioxidant enzyme activities, and some other cardinal metabolites and could be related to the difference in the mechanisms underlying oxidative emphasis hurt and subsequent tolerance to salt. These antioxidants can be used as possible choice standards for salt tolerance in mustard cultivars.