Nizatidine is H2 receptor adversary. It is widely prescribed as stomachic ulcer, duodenal ulcers. It has short biological half life and it is susceptible to metabolized by colonic bacteriums. The current probe concerns preparation of Nizatidine gastro recollective tablets by effervescent technique to increase the stomachic keeping clip and command the drug release up to 12 hour, besides better the bioavailability. In this field, optimized concentration of Na hydrogen carbonate ( 10 % ) used as gas generating agent. Release retarding agents used in this scrutiny are natural polyoses like Xanthane gum, pectin, Na alginate and man-made polymers like HPMC K100M. Each polymer used in concentration scope of 20 % , 25 % , 30 % , 35 % , 40 % to blockade the drug release. All prepared preparations were evaluated for weight fluctuation, hardness, crumbliness, drug content, In vitro perkiness surveies, swelling surveies and Invitro drug release surveies are performed. Except Na alginate comprised preparation all preparations exhibits less than 1 minute of perkiness slowdown clip farther more retain the matrix unity up to 12hours. Invitro drug release surveies displays that pectin contains preparation shows better drug release than other polymers. Drug release dynamicss of all preparations shows that they follow non Fickian diffusion mechanism to let go of the drug from prepared matrix tablets.


Oral path of disposal is most convenient manner of drug bringing in associate with better patent conformity as compared with other path of disposal and more than 60 % of marketed pharmaceutical merchandises are administered through unwritten path ( 1 ) . Over past three decennaries unwritten sustained release drug bringing systems have been gained a singular importance due to their curative advantages every bit good as patient conformity ( 2 ) .

The existent difference in planing of unwritten controlled release dose signifiers is non merely to retard the drug release for drawn-out period of clip besides retain the dose signifier in GI piece of land until the drug is wholly release from dose signifier. Most of orally administered sustained release dose signifiers are displacing from GI piece of land before let go ofing the drug from dose signifier due to faster stomachic emptying. In order to get the better of this sort of hardships, a important involvement is attained to develop gastro recollective dose from fast few decennaries ( 3 ) .

Gastric emptying surveies are unwrap that orally administered dose signifiers are significantly enduring from unpredictable stomachic emptying rate and short stomachic abode clip. Gastro recollective dose signifiers are able to protract the stomachic abode clip and avoid the job of short stomachic abode clip by floaty the dose signifier in tummy for prolong clip period ( 4 ) .

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The present probe is to develop Nizatidine gastroretentive tablets by effervescent technique to avoid the obstruction of short biological half life ( 1.5 to 2 hour ) , better the unwritten bioavailability every bit good as Nizatidine acts locally to handle stomachic and duodenal ulcers ( 5 ) .

Nizatidine is histamine H2-receptor adversary. It inhibit acerb production by reversibly viing with histamine for adhering to H2 receptors on the basolateral membrane of parietal cells. These drugs are less powerful than proton pump inhibitors but still suppress 24-hour stomachic acid secernment by about 70 % ( 6 ) .It is widely prescribed in stomachic ulcers, duodenal ulcers, Zollinger- Ellison syndrome and gastroesophageal reflux disease ( GERD ) .

Nizatidine was ready to metabolise by colonic bacteriums, which reduces the curative efficiency of Nizatidine. It does non bring on any anti-androgenic effects and drug interactions in patients as compared to any other category of H2 Receptor Antagonists ( 7 ) . Therefore, it is moderately better manner to better the curative efficaciousness of the drug when it is formulated as gastro recollective dose signifier.

In order to fix successful gastro recollective tablets of Nizatidine, optimized concentration of Na carbonate ( 10 % ) was incorporated which liberates C dioxide gas ( Co2 ) up on contact with stomachic juice nowadays in tummy, there by tablet denseness was reduced to below 1.004gm/cm3then tablet become floaty ( ( 8 ) . Release retarding polymers used in present probe were natural polyoses like Xanthane gum, Pectin, Sodium alginate and man-made polymer of HPMC K100M to blockade the drug release up to 12 hour ( 9 ) .

Inspite reaching of many man-made polymers, incorporation of natural polymer in gastro recollective dose signifier gaining batch of importance couple to their distinguished features of compatibility, cheap, ready handiness, safe and gel forming nature. Because of this illation present work is chiefly focused on natural polyoses ( 10 ) .

Materials and methods


Nizatidine pure drug was kindly supplied by shasun chemicals and drugs, Chennai as a gift sample. Xanthane gum, pectin were gifted by limpid colloids, Mumbai. HPMC K100M obtained as gift sample from Scope ingredients pvt Ltd, Chennai. Sodium hydrogen carbonate was gifted by Rishi chemical plants, Kolkata. Super tab 14SD as a trade name of Spray dried lactose provided by DFE drug company, Bangalore.


Preparation of Nizatidine gastro recollective tablets

Nizatidine gastro recollective tablets were prepared by direct compaction technique. The several pulverizations viz. Nizatidine, Let go ofing retarding polymers ( Xanthane gum, pectin, Na alginate, HPMC K100M ) , gas generating agent ( sodium hydrogen carbonate ) , dilutants ( spray dried lactose ) were passed through screen no. 60, individually. Then blending of pulverizations was carried out by utilizing stamp, motor for 5 batchs. Talc and Mg stearate were so added to the assorted pulverization. Mix was continued for another 3 batchs. Finally 400mg of each mixture were weighed and fed manually in to the dice of tablet compaction machine, equipped with level faced clouts ( 10mm ) to bring forth desire tablets ( 11,12 ) .

In vitro word picture of prepared tablets

Prepared tablets were tested for weight fluctuation, thickness measured by vernire callipers, hardness determined by Monsanto hardness examiner, By utilizing roche friabulator crumbliness was determined, all values are expressed as mean values A± SD ( 13, 14 ) .

Drug content

Accurately weighed equivalent to 15 milligram of Nizatidine transferred to a 50 milliliter volumetric flask dissolve in sufficient measure of methyl alcohol so do up the concluding volume with methyl alcohol and mix. Then filter the solution, collect the filtrate and made suited dilutions with methyl alcohol, so mensurate the optical density by utilizing UV seeable spectrophotometer at I»max of 320nm wavelength, there by calculate the sum of drug being in tablet ( 15 )

In vitro perkiness surveies:

In vitro perkiness surveies include perkiness slowdown clip, drifting continuance clip.

Buoyancy slowdown clip trial was performed to find whether the tablets were float on disintegration medium or non. In this trial the tablet was assigned on beaker consist of 200ml of 0.N HCl, so detect visually how much clip is taken by tablets to emerge on the surface of disintegration medium i.e. 0.1N HCl.

Floating continuance clip was determined by puting the tablets on beaker includes 200ml of 0.N HCl, so reason how much clip taken by tablet to possess on surface of 0.1N HCl medium ( 3,15,16 ) .

Swelling surveies:

A Tablet was weighed ( W1 ) and placed in a glass beaker, incorporating 200 milliliter of 0.1 N HCl. At regular clip intervals, the tablet were displaced and the extra surface liquid was carefully removed by a filter paper. The conceited tablet was so reweighed ( W2 ) . The swelling index ( SI ) was calculated utilizing below expression ( 17, 18 ) .

Swelling index =A-100

In vitro drug release surveies:

In vitro drug release surveies were carried out by USP TYPE II disintegration examiner setup ( paddle type ) . 0.1N HCl was taken as a disintegration medium which was placed on beakers of setup and maintained the temperature 37+ 0.50c. The paddles are allowed to revolve at a velocity of 50 revolutions per minute, so tablet was placed on beaker incorporating disintegration medium. Aliquots of 5 milliliters were detach from the disintegration setup at 0.25, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 hour clip intervals, At each clip of backdown, 5 milliliter of fresh medium was replaced into the disintegration flask. Then the drug content was determined spectrophotometrically at I»max of 228 nm wavelength ( 19 ) .

Drug release dynamicss

In order to analyze dynamicss of drug release, the obtained information was analyzed by different theoretical account dependent dynamicss. Tease surveies are based on different mathematical maps, which describe the disintegration profile. The theoretical account dependent attacks include zero order ( 20 ) , First order, Higuchi, korsmeyer-peppas theoretical accounts ( 21, 22 ) .

Drug – Excipient interaction surveies

Drug – Excipient interaction are determined by Fourier transform infrared ( FT IR ) spectrometry. As a portion of this work, KBr phonograph record method was preferred to enter the spectra of samples. In this manner each sample was gently triturated with recrystalized KBr pulverization in proportion of 1:100 so pressed by hydrostatic imperativeness to organize phonograph record. Then the phonograph record was placed on sample holder scanned from 4000 to 400cm-1, so spectra of Nizatidine pure drug, Xanthane gum, pectin, Na alginate, HPMC K100M and optimized preparation ( Nizatidine & A ; pectin ) was recorded ( 23 ) .

Tablet readying for In vivo surveies

All the ingredients used in readying of Nizatidine gastro recollective tablets were crystalline to X raies. In order to opaque the administered tablet in tummy by X raies, incorporation of wireless contrast agent is necessary. Barium Sulphate ( BaSO4 ) is most frequently used in imagination of the GI piece of land. but it has high denseness ( 4.4777 gram /cm3 ) and hapless drifting belongings. So 12 % concentration of BaSO4 is enclosed in tablet on behalf of 12 % of Nizatidine to guarantee the tablet float on tummy. In add-on to BaSO4, 30 % pectin, 5 % PVP K30, 2 % Talc, 1 % Mg stearate were accurately weigh and mix to organize unvarying blend so compress by utilizing tablet compaction machine.

In vivo surveies was carried out in 3 healthy topics. Subjects were allowed to fasted for nightlong. BaSO4 enclosed prepared tablets were administered to topics were allowed to expose X radiations to acquire abdominal X beam imagination. At regular clip intervals of 0.15, 2,4,6,8 hour, X beam images were taken in standing place of topics by keeping changeless distance between X- beams and topics. Gastric recollective clip of Nizatidine gastro recollective tablets was estimated from the images of X- beam skiagraphy surveies ( 24 ) .

Consequences and treatment

In the present survey nizatidine gastro recollective tablets were favourably formulated by utilizing different ratios of Xanthane gum, pectin, Na alginate, and HPMC K100M along with Na hydrogen carbonate as gas generating agent.

Matrix tablet word picture

The Nizatidine gastro recollective tablets were prepared have off white, smooth, and level shaped in visual aspect. All the prepared tablets shows agreeable weight fluctuation scope of 397 to 405mg with standard divergence scope of & lt ; 3, and non more than two tablets were allowed to divert from per centum weight fluctuation scope as specified in Indian pharmacopoeia ( IP ) . Thickness of prepared tablets were measured by varnier callipers. It was shown between the scope of 3.2A±0.05 to 3.4A±0.46. Hardness of prepared tablets shows between the scope of 3.8A±0.96 to 4.46A±0.115 with standard divergence of & lt ; 2.

In vitro perkiness surveies

All preparations of Nizatidine gastro recollective tablets were prepared by effervescent technique. In all preparations optimized concentration ( 10 % ) of Na hydrogen carbonate used as effervescent agent which induces perkiness to prepared tablets without set uping matrix unity for up to 12 hours, with maximal possible slowdown clip.

In acerb environment Na hydrogen carbonate release C dioxide gas which was enclosed and protected with gel barrier formed by swelling of hydrated barrier, therefore the tablet denseness decreased to below 1.004gm/cm3. As decreases the tablet denseness tablet go floaty.

Buoyancy survey values of all preparations are mentioned in tabular array. Formulations prepared with Xanthane gum, pectin, and HPMC K100M shows perkiness slowdown clip & lt ; 1 minuet with standard divergence scope of & lt ; 2 and retain the matrix unity about 12 hour. But preparation prepared with Na alginate shows drifting lag clip 1 to 2 proceedingss and failed to retain matrix unity up to 12 hours as low swelling rate of polymer unable to organize gel barrier.

Swelling survey

Swelling index is one of the first factor, plays a meaningful function to guarantee perkiness to prepared tablets every bit good as to bear matrix unity up to coveted period. Based on swelling nature of polymer used tablet, gel bed is formed which represents the drug release.

Swelling index values of all preparation were expressed in tabular array. In instance of tablet prepared with Xanthane gum ( F1 to F5 ) , HPMC K100M ( F15 to F20 ) swelling index values are bit by bit increase up to 12 hour, relatively preparation prepared with HPMC K100M shows high swelling index value than other polymers. Indeed, Tablets prepared with pectin ( F6to F10 ) exhibits swelling index values are bit by bit increase in first 6 hour than somewhat decreases because rapid hydration of polymer in initial hours. Formulations prepared with Na alginate ( F11 to F15 ) shows low swelling index values because it has hapless swelling and hydration nature. Graphic representation of swelling index values of all preparations are shown in fig.

Invitro disintegration survey

In vitro disintegration survey was carried out in 0.1N HCl disintegration medium utilizing usp type II disintegration setup, disintegration was carried out up to 12hrs by retreating samples every one hr.

In vitro drug release values of Nizatidine gastro recollective tablets are mentioned in table no. Formulations prepared with 20 % concentration of xanthane gum ( F1 ) release 99.57 % of drug with in 10 hours. 25 % concentration of xanthane gum ( F2 ) release98.7 % of drug in 11 hours. But staying 30 % ( F3 ) , 35 % ( F4 ) , 40 % ( F5 ) concentrations Xanthane gum release 97.9 % ,97.2 % , 95.97 % of drug severally at 12th hr. Which denotes that the 20 % , 25 % concentration of Xanthane gum was non acceptable to blockade the drug release up to 12 hours.

Formulation developed with 20 % ( F6 ) , 25 % ( F7 ) concentration of pectin exhibits 99.26 % , 98.72 % of drug release up to 11 hour. whereas 30 % ( F8 ) , 35 % ( F9 ) , 40 % ( F10 ) concentrations of pectin shows 99.1 % , 97.38 % , 96.78 % of drug release severally. Among all preparations of F6 to F10, Formulation prepared with 30 % concentration of pectin show better drug release, so it is desire concentration to acquire Nizatidine drifting tablets.

Formulations prepared with 20 % to 35 % ( F11 to F14 ) concentration of Na alginate failed to retard the drug release up to 12 hour because it have limited swelling and low gel organizing belongings. Formulations prepared with HPMC K100M shows really slow drug release, unable to present 70 % of drug in 8hrs. But 98 % , 97.3 % , 96 % , 96.7 % , 94.4 % of drug release was shown by preparation contain 20 % , 25 % ,30 % ,35 % ,40 % concentrations of HPMC K100M. this is due to HPMC K100M produce high viscousness of gel barrier around the tablet which takes longer clip to gnaw. Among all polymers pectin enclosed preparation shows better drug release than others, this may be due to increase the solubility of meagerly soluble Nizatidine by pectin which can able to increase the solubility. Graphic Representation of in vitro drug release profile of all preparations is shown in fig.

Drug release dynamicss

To understand the drug release dynamicss and mechanism of drug release from prepared tablets in vitro drug release informations was analyzed by different theoretical account depend dynamicss like zero order, First order, Higuchi, krosmeyer-peppas theoretical accounts. Regression coefficient ( R2 ) values obtained by kinetic theoretical accounts are illustrates in tabular array. Dissolution profile of most of preparations follows zero order dynamicss, preparation prepared with 35 % of xanthane gum, 30 % of pectin arrested development values shows additive drug release profile. From the kinetic informations of krosemeyer – peppas theoretical account, we can corroborate the mechanism of drug release. In this theoretical account release exponent ‘n ‘ explains the mechanism of drug release from prepared tablets. If n values is & lt ; 0.45, that follows Fickian diffusion. Similarly, n value is in between 0.45 -0.89 which follows non Fickian conveyance mechanism. If ‘n ‘ is More than 0.89 which follows instance II conveyance. As for this present work concerns, drug release profile of all preparations follows non Fickian conveyance to let go of the drug from prepared tablets.

In vivo drifting behaviour

In vivo drifting behaviour of prepared gastro recollective tablets were observed by radiographic imaging technique in 3 healthy topics. Tablets prepared with 12 % concentration of BaSo4 can able to opaque in X-ray image which illation that 12 % concentration of BaSo4 is adequate to fix tablet for In vivo X-ray imagination surveies to foretell the stomachic keeping clip.

Tablet can able to visualise in x-ray image taken in 0.15hr indicates that the administered tablet was float on tummy with in 15 minuts. X beam images taken in 2nd,4th and 6th hr unwrap that the tablet being in tummy up to 6hrs and change in tablet place reveals that the tablet did non adhere to stomach mucous membrane.



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