Which enzyme(s) will produce a DNA fragment that contains the entire vgp gene (shown in red) and has “sticky ends”?
*BamHI

*HindIII

Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene?
BamHI, HaeIII, and HindIII
Which enzyme(s) would cut the plasmid without disrupting the function of the ampR gene?
BamHI, EcoRI, and HaeIII
Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid?
BamHI, EcoRI, and HindIII
Which one restriction enzyme satisfies all three of the requirements listed above?
BamHI only
Will only grow in medium without ampicillin
No plasmid
Will grow in both media
*Recombinant plasmid with vgp gene

*Nonrecombiant plasmid

*Recombinant plasmid but no vgp gene

Which answer best explains why restriction endonucleases like EcoRI create DNA fragments with sticky ends?
They make a staggered cut within the recognition site by cutting the two DNA strands at different locations.
For the pituitary gland cDNA library shown in Figure 20.4, is each type of cDNA in the library likely to be represented just once? Why or why not?
No, because each cDNA is likely to be present in more than one copy after mRNA isolation and cDNA synthesis.
In gene-cloning projects, plasmids function to ______.
allow replication of the DNA that’s being cloned
What defect causes pituitary dwarfism?
Lack of growth hormone
What is genetic cloning?
A method to produce many copies of a gene
True or false? The use of growth hormone isolated from cadavers was banned mainly because there were not enough cadavers to supply the hormone.
False
Which of the following statements best describes the function of reverse transcriptase?
It makes complementary DNA (cDNA) from mRNA.
Which of the following steps would be performed last when screening a cDNA library?
Match the region of radioactivity on the filter with the corresponding plate.
How can large quantities of protein be produced from a bacterial colony containing the gene of interest?
Put a cDNA encoding the protein into a plasmid with a bacterial promoter sequence.
True or false? The term “sticky ends” refers to the overhanging ends on DNA that are generated by restriction enzymes, which can base pair with any DNA molecules that contain complementary sticky ends.
True
The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
sticky ends
The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.
-ACGT
In order to insert a human gene into a plasmid, both must _____
be cut by the same restriction enzyme
What enzyme forms covalent bonds between restriction fragments?
DNA ligase
What name is given to the process shown in this animation?
transformation
What name is given to the process shown in this animation?
cloning
In this example the marker DNA includes fragments that have 23,130, 9,416, 6,557, 4,361, 2,322, 2,027, and 564 base pairs. Approximately how many base pairs are in the DNA fragment indicated by the letter A?
6,557
In this example the marker DNA includes fragments that have 23,130, 9,416, 6,557, 4,361, 2,322, 2,027, and 564 base pairs. Approximately how many base pairs are in the DNA fragment indicated by the letter B?
between 6,557 and 4,361 base pairs
DNA fragment B consists of _____ base pairs.
1,405
Which of these DNA molecules is the shortest?
E
In gel electrophoresis DNA molecules migrate from _____ to _____ ends of the gel.
negative … positive
In the genetic engineering of plants, plants are exposed to the bacterium Agrobacterium tumefaciens to ______.
introduce engineered DNA into plant cells
How are foreign genes inserted into plants?
T DNA containing foreign DNA sequences is inserted into plant-cell chromosomes.
If a particular sequence of DNA were amplified using 15 PCR cycles, then the amount of this DNA would be predicted to increase by _____ -fold.
3.28?10^4
x

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