The Effect of Alcohol and Caffeine on the Water Flea: Daphnia Magna Kristoffer Osuntuyi Daphnia Magna Abstract: In this experiment I will find the base heart rate of each water flea. The base heart rate is the control in my experiment. The variables in the experiment are alcohol and caffeine. After finding the control of the first flea I will surround it with alcohol. The second flea will go through the same process as the first flea did, except the variable will be caffeine. The purpose of these two experiments is to see how surrounding one water flea with alcohol and another with caffeine affects each water flea’s heart rate.

I believe the Daphnia Magnus (two different water fleas) will go through a similar experience and similar side effects of having alcohol and caffeine in their bodies as humans do. In this experiment a drop of 2%, 4%, and 6% of alcohol will be administered to the first Daphnia Magna. The second Daphnia Magna will have a drop of 1%, 1. 5%, and 2% of caffeine administered. Daphnia Magna have a single compound eye, two branched antennae, and they look almost kidney-shaped. They also have a carapace, which is the upper section of the water flea’s external shell.

The water flea has leaf-like limbs, in which they produce a current of water and filter food and oxygen to the mouth and its gills. According to some of my research, talking to my father, the Daphnia has a relatively low percentage of body fat; this may affect them the most when surrounded by alcohol. Daphnia Hypothesis: It is expected that the effects of the higher percent doses of alcohol and caffeine will have the greatest effect on the Daphnia in comparison to the lower percentages.

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The results will show that there is a limit to how much alcohol and caffeine the Daphnia can withstand before needing the use a control, crashing, or even dying. I believe this to be true because similar symptoms of ingesting alcohol and caffeine happen in us humans. Materials and Methods used in our Experiment: In this experiment I used a microscope to view each of the water fleas on a microscope slide. A dropper was used to place the Daphnia Magna onto the slide in pure water, the control. A paper towel was used to remove the water from the slide.

I used this to be able to place the drops of 2%, 4%, and 6% alcoholic solution on the flea, and also to place the drops of 1%, 1. 5%, and 2% caffeine solution on the second flea. I used the timer on my phone watch to count the heartbeats and to make sure the solution was on the Daphnia for a minute before counting the heart rate. Each water flea experiment was viewed under a 40x magnification. Daphnia heart rate with Alcohol Results: Before introducing the Daphnia (water flea) to three different series of alcohol concentration, I took an initial heart rate.

The heart rate of the water flea in pond water was an average of 216 beats per minute. When introduced to a concentration level of two percent the average heart decreased to 132 beats per minute. The second concentration level was four percent the average heart decreased to an average of 96 heart beats per minute. The third concentration level was six percent and when introduced to this final solution the Daphnia heart plummeted to 76 beats per minute. After the testing the water flea was returned to pond water and lived. Daphnia heart rate with caffeine Results:

Before introducing the Daphnia (water flea) to three different series of caffeine concentration, I took an initial heart rate. This water flea was a different from the initial water flea in the alcohol concentration experiment. The heart rate of the water flea in pond water was an average of 224 beats per minute. When introduced to a concentration level of one percent the average heart increased to 288 beats per minute. The second concentration level was one and a half percent the average heart decreased to an average of 284 beats per minute.

The third concentration level was two percent and when introduced to this final solution the Daphnia heart plummeted to 216 beats per minute. After the testing the water flea later died. Post Experiment Discussion on the Results: The purpose of this experiment was to determine the effect of alcohol and caffeine on Daphnia Magna. The alcohol slowed the heart rate down because it is a depressant. It affected the Daphnia in the same way that it would a human. The higher the percentage of alcohol, the slower the Daphnia’s movement and reaction time was until it came close to death.

When the Daphnia was exposed to the caffeine, the its heart rate increased due to the caffeine being a stimulant. It had the same effect as that of a human drinking a cup of coffee or an energy drink (i. e. Monster). The result of the drop of caffeine to the Daphnia enabled its heart rate to rise, causing it to stay alive longer than the first alcohol surrounded water flea. I believe if I would have added 8% and or even 10% of alcohol to the water flea, my results and graph would continue to decrease. Eventually the water flea would have died. The water flea would have probably come close to dying at 8%, and it would definitely die at 10%.

This opinion is formulated from seeing that the flea has similar effects to us humans, too much alcohol and we will come pretty close to death or even die. Unfortunately for our second flea surrounded by caffeine it died. If it did not die I predict that adding higher doses of caffeine to the flea my results would show that there is a limit on how much caffeine it could take before a complete crash of the organism’s heart crashes and eventually come close to death, or dies. Again, this opinion was formulated due to the similar effects that caffeine has on us humans. Excessive alcohol consumption will cause alcohol poisoning.

This condition is extremely dangerous and is fatal. Alcohol poisoning occurs when a large amount of alcohol is consumed, such as drinking large amounts within a short amount of time. The age, size, and weight of the person can determine how much alcohol consumption is okay before the poisoning process occurs. If we intake too much caffeine, our breathing, heart rate, and blood flow may quicken. This effect can lead to high blood pressure, dizziness, sweating, shaking, and tremors. Mentally and emotionally, we may experience nervousness, jitteriness, irritability, anxiety, stress and agitation.

Too much caffeine also affects our ability to sleep, causing restlessness, insomnia, and sleep loss. In more serious cases of caffeine overdose, we may experience trouble breathing, fever, vomiting, mental confusion, or even hallucinations. Our increased heart rate may lead to a dangerously rapid, slow or irregular heartbeat. At the extreme, these effects can cause cardiac arrest, coma, and even death. Citation: Stephanie Puckett eHow Contributor, http://www. ehow. com/how-does_5557651_happens-drink-much-alcohol. html, What Happens When You Drink Too Much Alcohol?

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